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human brain hippocampus whole tissue lysates
Human Brain Hippocampus Whole Tissue Lysates, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+hippocampus+whole+tissue+lysates/Human+Brain+Hippocampus+Whole+Tissue+Lysate+(Adult+Whole+Normal)/pm38926592-125-14-30
Average 92 stars, based on 2 article reviews
Human Brain Hippocampus Whole Tissue Lysates, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+hippocampus+whole+tissue+lysates/Human+Brain+Hippocampus+Whole+Tissue+Lysate+(Adult+Whole+Normal)/pm38926592-125-14-30
Average 92 stars, based on 2 article reviews
human brain hippocampus whole tissue lysates - by Bioz Stars,
2026-09
92/100 stars
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1) Product Images from "Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients."
Article Title: Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients.
Journal: Scientific reports
doi: 10.1038/s41598-024-65608-x
Figure Legend Snippet: Figure 3. A polyclonal N-terminal ADNP antibody from Aviva Systems detects ADNP specifically in murine and rat tissues and suggests proteolytic processing of the protein in the human brain. Cerebellum, frontal cortex or lobe, hippocampus and whole brains of control mice, rats and humans were lysed in RIPA buffer and used as protein samples for the assessment of N-terminal antibody of Aviva systems. (A–C) The predicted molecular weight of ADNP is 124 kDa. The antibody recognizes ADNP in a range of 145 kDa with (E) additional lower mass signal of 85 kDa in all human brain regions. (B–D–F) Western blot analysis of the blocking peptide competition assay. Supplementation of the immunization peptide in a 5 × excess to antibody concentration reduced the signal observed at 145 kDa in all tested cell lines. Importantly, the 85 kDa band suggestive for proteolytic cleavage as well as degraded ADNP signal disappeared completely after immunization peptide supplementation. GAPDH was used as a loading control.
Techniques Used: Control, Molecular Weight, Western Blot, Blocking Assay, Competitive Binding Assay, Concentration Assay
Figure Legend Snippet: Figure 5. Different C-terminal ADNP antibodies detect ADNP in the range of 150 kDa and suggest proteolytic processing of the protein in the brain. Cerebellum, frontal cortex or lobe, hippocampus and whole brains of control mice, rats, and humans were lysed in RIPA buffer and used as protein samples for the assessment with three C-terminal antibodies with the optimized dilutions listed in Table 3. GAPDH was used as a loading control. The predicted molecular weight of ADNP is 124 kDa. (A)C) Murine samples indicate detection of ADNP in the range of 150 kDa with bands suggesting proteolytic processing at 50 kDa. (D–F) Rat samples indicate detection of ADNP in the range of 150 kDa with bands indicating proteolytic processing at 82 kDa after incubation with the C-terminal Abcam antibody. (G–I) Human brain samples indicate detection of ADNP at different molecular weights of 124 – 150 kDa in the adult frontal lobe and hippocampus and highlight the antibody differences in detection of ADNP. The three tested antibodies showed strong band signals at lower molecular weights, which could indicate proteolytic cleavage or degradation of the protein.
Techniques Used: Control, Molecular Weight, Incubation
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Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB82059186), and human brain hippocampus whole tissue Article Title: Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: Tracing the Invisible Mutant ADNP protein in Helsmoortel- Van der Aa Syndrome patients Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. 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Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB82059186), and human brain hippocampus whole tissue Article Title: Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: Tracing the Invisible Mutant ADNP protein in Helsmoortel- Van der Aa Syndrome patients Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. Post-mortem tissues and subjects Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: In Search of the Hidden Protein: Optimization of Detection Strategies for autism-associated Activity-Dependent Neuroprotective Protein (ADNP) mutants. Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. 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Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB82059186), and human brain hippocampus whole tissue Article Title: Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: Tracing the Invisible Mutant ADNP protein in Helsmoortel- Van der Aa Syndrome patients Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. Post-mortem tissues and subjects Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: In Search of the Hidden Protein: Optimization of Detection Strategies for autism-associated Activity-Dependent Neuroprotective Protein (ADNP) mutants. Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. Post-mortem tissues and subjects Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820-59186), and human brain hippocampus whole tissue lysates ( Construct:Article Title: Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients. Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB82059186), and human brain hippocampus whole tissue Article Title: Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients Article Snippet: Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: Tracing the Invisible Mutant ADNP protein in Helsmoortel- Van der Aa Syndrome patients Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. Post-mortem tissues and subjects Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820- 59186), and human brain hippocampus whole tissue lysates ( Article Title: In Search of the Hidden Protein: Optimization of Detection Strategies for autism-associated Activity-Dependent Neuroprotective Protein (ADNP) mutants. Article Snippet: All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.All conducted experiments were in compliance with the EU Directive 20120/63/EU under ECD code 2020-14 after approval by the Animal Ethics Committee of the University of Antwerp.. Post-mortem tissues and subjects Adult human brain (NB820-59177), human brain cerebellum (NB820-59180), human brain frontal lobe (NB820-59186), and human brain hippocampus whole tissue lysates ( |